Long-Read sequencing of E. coli: Ghanaian patient - SRA

SRX14720811: Long-Read sequencing of E. coli: Ghanaian patient
1 OXFORD_NANOPORE (PromethION) run: 188,704 spots, 3G bases, 2.4Gb downloads

Design: Isolated DNA was sent for sequencing to BGI Genomics. Libraries were prepared at the sequencing facility. Isolates were sequenced on a R9.4.1 flowcell with a Oxford Nanopore PromethION device. The HAC basecalling model was used. Reads shorter than 2000bp were filtered out.

Submitted by: University Medical Center Goettingen (UMG)

Study: Microepidemiology of plasmids in Escherichia coli and Klebsiella pneumoniae with acquired extended spectrum beta-lactamase genes isolated from chronic wounds in Ghana

PRJNA822931 • SRP367404 • All experiments • All runs

show Abstracthide Abstract

Little information on the microepidemiology of mobile genetic elements like plasmids harboring acquired beta-lactamase genes in Western African Ghana is available so far. In the study presented here, we screened for plasmids in 3 Escherichia coli and 4 Klebsiella pneumoniae isolates from chronically infected wounds of Ghanaian patients expressing extended spectrum beta-lactamases (ESBL) mediated by the blaCTX-M-15 gene. The bacterial isolates were subjected to combined short-read and long-read sequencing to obtain the sequences of their plasmids

Sample: BNI_Iso00041

SAMN17766542 • SRS8179259 • All experiments • All runs

Organism: Escherichia coli

Library:

Name: Iso00041_ONP

Instrument: PromethION

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 188,704 spots, 3G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR18592171	188,704	3G	2.4Gb	2022-04-12

ID:: 21076430

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